prospec

POLR2J3 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • POLR2J3 Human
  • Polymerase II Polypeptide J3 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-654

Synonyms

Polymerase (RNA) II (DNA Directed) Polypeptide J3, DNA-Directed RNA Polymerase II Subunit 11, DNA-Directed RNA Polymerase II Subunit RPB11-B2, DNA-Directed RNA Polymerase II Subunit J3, RNA Polymerase II Subunit B11-B2, POLR2J2, RPB11b2, RPB11b1.

Introduction

DNA-directed RNA polymerase II subunit RPB11-b2 (POLR2J3) is a member of the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exists in 3 variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Description

POLR2J3 Human Recombinant produced in E. coli is a single polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5 kDa.
POLR2J3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The POLR2J3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

Purity

Greater than 95% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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