Profilin-1, Allergen Phl p 11, Pollen allergen Phl p 12, Phl p 12, PRO1, PHLPXI.
Phl p 12.0101 a profilin, is a minor grass pollen allergen which is involved in cytoskeleton mobility by interacting with actin filaments. It is well recognized that IgE binding can cause immune cross-reactivity with profilins of plant-derived foods and pollen profilin.
Recombinant PhI p 12 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 15,607 Dalton.
PhI p 12 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
PhI p 12 is supplied in 20mM HEPES buffer pH-7.5, 250mM NaCl and 20% glycerol.
Avoid multiple freeze-thaw cycles.
2. Immunodot test with positive/negative sera panels.