Pfu-ssod7 DNA Polymerase

Pfu-ssod7 DNA Polymerase is derived from E. coli, having a molecular mass of 100 kDa. The Pfu-ssod7 DNA Polymerase possesses the following activities: 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity. It generates blunt ends in the amplification products. The Pfu-ssod7 DNA Polymerase brings together a Pyrococcus furiosus DNA polymerase with a proc+essivity-enhancing domain Ssod7.

20mM Tris-HCl (pH 8.0), 50% glycerol, 0.1mM EDTA, 100mM KCl, 1mM DTT, 0.1% Tween20 and 0.1% NP-40.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

1U of enzyme catalyzes the incorporation of 10nmol of dNTP into polynucleotide fraction in 30 minutes at 74°C.

200mM Tris-HCl (pH 8.8), 100mM (NH4)2SO4, 1mg/mL BSA, 100mM KCl, 20mM MgSO4, 1% Triton X-100.

Greater than 95 % as determined by SDS-PAGE analyses

It is applicable for amplification reaction of genomic DNA, cDNA, dU-containing DNA and crude samples as templates.

SDS

The Pfu-ssod7 DNA Polymerase preforms very well for all major PCR applications. The Pfu-ssod7 DNA Polymerase generates long amplicons with accuracy and speed. The high fidelity makes the Pfu-ssod7 DNA Polymerase a great choice for cloning. The error rate of Pfu-ssod7 DNA Polymerase is approximately 50 fold lower than that of the Thermus aquaticus DNA polymerase. It generates blunt ends in the amplification products.