prospec

NT 4 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • NT 4 Human
  • Neurotrophin-4 Human Recombinant
  • Shipped at Room temp.

Catalogue number

CYT-626

Synonyms

NT4, NT5, NTF5, NT-4/5, NTF4, Neurotrophin-4, Neutrophic factor 4, Neurotrophin-5, NT-5.

Introduction

NT-4 is part of the family of neurotrophic factors, neurotrophins, that are in charge for the survival and differentiation of mammalian neurons. NT-4 expression is dominant and less influenced by environmental signals. NT-4 deficient mice shows slight cellular deficits and develop normally to adulthood. NT-4 is a target-derived survival factor for peripheral sensory sympathetic neurons.NT-4 is involved in the proliferation and differentiation of periodontal ligament cells.

Description

Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa.
The NT-4 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM phosphate buffer pH-7.4 and 150mM NaCl.

Solubility

It is recommended to reconstitute the lyophilized NT-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized NT-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NT-4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence

GVSETAPASR RGELAVCDAV SGWVTDRRTA VDLRGREVEV LGEVPAAGGS PLRQYFFETR CKADNAEEGG PGAGGGGCRG VDRRHWVSEC KAKQSYVRAL TADAQGRVGW RWIRIDTACV CTLLSRTGRA.

Biological Activity

Determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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