prospec

NMT1 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • NMT1 Human
  • N-Myristoyltransferase 1 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-842

Synonyms

N-Myristoyltransferase 1, NMT, Myristoyl-CoA:Protein N-Myristoyltransferase 1, Type I N-Myristoyltransferase, EC 2.3.1.97, Myristoyl-CoA:Protein,N- Myristoyltransferase, Glycylpeptide N-Tetradecanoyltransferase 1,Peptide N-Myristoyltransferase 1,Alternative, Short Form NMT-S, Short Form NMT-S,Long Form, NMT-L, Alternative, Long Form, NMT-L, NMT 1.

Introduction

Myristate, a rare 14-carbon saturated fatty acid, is co-translationally attached by an amide linkage to the N-terminal glycine residue of cellular & viral proteins with various functions. N-Myristoyltransferase 1, also known as NMT1 catalyzes the transfer of myristate from CoA  to proteins. NMT1 seems to be irreversible and is essential for full expression of the biologic activities of several N-myristoylated proteins, as well as the alpha subunit of the signal-transducing guanine nucleotide-binding protein, G protein.

Description

NMT1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 519 amino acids (1-496 a.a) and having a molecular mass of 59.2kDa.
NMT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

NMT1 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4).

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMADESET AVKPPAPPLP QMMEGNGNGH EHCSDCENEE DNSYNRGGLS PANDTGAKKK KKKQKKKKEK GSETDSAQDQ PVKMNSLPAE RIQEIQKAIE LFSVGQGPAK TMEEASKRSY QFWDTQPVPK LGEVVNTHGP VEPDKDNIRQ EPYTLPQGFT WDALDLGDRG VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL PQWHCGVRVV SSRKLVGFIS AIPANIHIYD TEKKMVEINF LCVHKKLRSK RVAPVLIREI TRRVHLEGIF QAVYTAGVVL PKPVGTCRYW HRSLNPRKLI EVKFSHLSRN MTMQRTMKLY RLPETPKTAG LRPMETKDIP VVHQLLTRYL KQFHLTPVMS QEEVEHWFYP QENIIDTFVV ENANGEVTDF LSFYTLPSTI MNHPTHKSLK AAYSFYNVHT QTPLLDLMSD ALVLAKMKGF DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWKCPSMG AEKVGLVLQ.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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