The NT-3 is purified by proprietary chromatographic techniques.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.
Safety Data Sheet
1.Title:Generation of Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant^†^‡.
Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.
2.Title:Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action.
Publication: Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008.
Molecular Therapy (2008) 16 11, 1873–1882 doi:10.1038/mt.2008.189
3.Title: TrkA Receptor “Hot Spots” for Binding of NT-3 as a Heterologous Ligand
Publications: Journal of Biological Chemistry 282.23 (2007): 16754-16763.
4.Title: Orphan nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanism
Publication: FEBS letters 583.9 (2009): 1505-1510