prospec

LYVE1 Human Sf9

  • Name
  • Description
  • Pricings
  • Quantity
  • LYVE1 Human Sf9
  • Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1 Human Recombinant, Sf9
  • Shipped at Room temp.

Catalogue number

PKA-250

Synonyms

Lymphatic vessel endothelial hyaluronic acid receptor 1 precursor, LYVE-1, Cell surface retention sequence-binding protein 1, CRSBP-1, Hyaluronic acid receptor, Extracellular link domain-containing protein 1.

Introduction

LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module the prototypic HA binding domain of the Link protein superfamily. Like CD44, the LYVE-1 molecule binds both soluble and immobilized HA. However, unlike CD44, the LYVE-1 molecule colocalizes with HA on the luminal face of the lymph vessel wall and is completely absent from blood vessels. Hence, LYVE-1 is the first lymph-specific HA receptor to be characterized and is a uniquely powerful marker for lymph vessels themselves.

Description

Soluble LYVE1 Human Recombinant fused to a C-terminal His-tag (6xHis) produced in baculovirus is a monomeric, glycosylated, polypeptide containing 232 amino acids (Met-1 to Gly 232) and having a total molecular mass of 25 kDa but as a result of glycosilation the Mw is 40 kDa.
The LYVE-1 is purified by proprietary chromatographic techniques.

Source

Insect Cells.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

LYVE1 was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives.

Solubility

It is recommended to reconstitute the lyophilized LYVE1 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized sLYVE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sLYVE-1 should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Back to Top