LRG1

LRG1

LRG1

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  • View Data Sheet

    Description:

    Leucine-Rich Alpha-2-Glycoprotein 1 Human Recombinant, Sf9

    Leucine Rich Alpha-2-Glycoprotein 1, Leucine-Rich Alpha-2-Glycoprotein, 1300008B03Rik, 2310031E04Rik, HMFT1766, Leucine-rich alpha-2-glycoprotein, LRG1, LRG. 

    Product # :

    PRO-2530

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    Description

    LRG1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 321 amino acids (36-347a.a.) and having a molecular mass of 35.4kDa. LRG1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

    Source

    Sf9, Baculovirus cells.

    Formulation

    LRG1 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

    Purity

    Greater than 95.0% as determined by SDS-PAGE.

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    • Introduction

      LRG1 belongs to the leucine-rich repeat (LRR) family of proteins, which have been shown to be involved in protein-protein interaction, signal transduction, cell adhesion and development. The LRG1 is expressed during granulocyte differentiation.

    • Synonyms

      Leucine Rich Alpha-2-Glycoprotein 1, Leucine-Rich Alpha-2-Glycoprotein, 1300008B03Rik, 2310031E04Rik, HMFT1766, Leucine-rich alpha-2-glycoprotein, LRG1, LRG.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      ADPVTLSPKD CQVFRSDHGS SISCQPPAEI PGYLPADTVH LAVEFFNLTH LPANLLQGAS KLQELHLSSN GLESLSPEFL RPVPQLRVLD LTRNALTGLP PGLFQASATL DTLVLKENQL EVLEVSWLHG LKALGHLDLS GNRLRKLPPG LLANFTLLRT LDLGENQLET LPPDLLRGPL QLERLHLEGN KLQVLGKDLL LPQPDLRYLF LNGNKLARVA AGAFQGLRQL DMLDLSNNSL ASVPEGLWAS LGQPNWDMRD GFDISGNPWI CDQNLSDLYR WLQAQKDKMF SQNDTRCAGP EAVKGQTLLA VAKSQHHHHH H

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lrg1 Protein
  • View Data Sheet

    Description:

    Leucine-Rich Alpha-2-Glycoprotein 1 Human Recombinant

    800x600 Leucine-rich alpha-2-glycoprotein, Leucine-Rich Alpha-2-Glycoprotein 1, LRG, LRG1, HMFT1766.

    Product # :

    PRO-1676

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    Description

    LRG1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 335 amino acids (36-347) and having a molecular mass of 36 kDa.LRG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

    Source

    Escherichia Coli.

    Formulation

    The LRG1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

    Purity

    Greater than 85.0% as determined by SDS-PAGE.

    More Info

    • Introduction

      LRG1 belongs to the leucine-rich repeat (LRR) family of proteins, which have been shown to be involved in protein-protein interaction, signal transduction, cell adhesion and development. The LRG1 is expressed during granulocyte differentiation.

    • Synonyms

      Leucine-rich alpha-2-glycoprotein, Leucine-Rich Alpha-2-Glycoprotein 1, LRG, LRG1, HMFT1766.

    • Physical Appearance

      Sterile Filtered colorless solution.

    • Stability

      Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

    • Amino Acid Sequence

      MGSSHHHHHH SSGLVPRGSH MGSVTLSPKD CQVFRSDHGS SISCQPPAEI PGYLPADTVH LAVEFFNLTH LPANLLQGAS KLQELHLSSN GLESLSPEFL RPVPQLRVLD LTRNALTGLP PGLFQASATL DTLVLKENQL EVLEVSWLHG LKALGHLDLS GNRLRKLPPG LLANFTLLRT LDLGENQLET LPPDLLRGPL QLERLHLEGN KLQVLGKDLL LPQPDLRYLF LNGNKLARVA AGAFQGLRQL DMLDLSNNSL ASVPEGLWAS LGQPNWDMRD GFDISGNPWI CDQNLSDLYR WLQAQKDKMF SQNDTRCAGP EAVKGQTLLA VAKSQ.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Human Lrg1
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    Description:

    Leucine-Rich Alpha-2-Glycoprotein 1 Canine

    Leucine-rich alpha-2-glycoprotein, LRG, LRG1, FLJ45787, HMFT1766.

    Product # :

    PRO-1592

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    Description

    The Canine LRG1 produced from pooled Canine Serum has a molecular mass of 38.129kDa (calculated without glycosylation) containing 347 amino acid residues.

    Source

    Canine Serum.

    Formulation

    LRG1 protein filtered (0.4µm) and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 8.0.

    Purity

    Purity greater than 90% as determined by SDS-PAGE.

    More Info

    • Introduction

      LRG1 belongs to the leucine-rich repeat (LRR) family of proteins, which have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. The LRG1 is expressed during granulocyte differentiation.

    • Synonyms

      Leucine-rich alpha-2-glycoprotein, LRG, LRG1, FLJ45787, HMFT1766.

    • Physical Appearance

      Filtered White lyophilized (freeze-dried) powder.

    • Stability

      Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

    • Solubility

      It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

    • Amino Acid Sequence

      MSPWSPERSQ SPGGLSSHLS KTLLLLLFSV ASAQGVTPDR DTCLVFPSSN GSSVSCHPPA KLPHHFPSDT VHLVVEFFNL TQLRPDTLQG VSNLQELHLS TNQLESLSPK LLLPVPLLKV LDLTRNALTQ LPPGLFRVSA ALHTLVLKEN RLEVLEPSWL CGLKALGHLD LSGNHLQTLP PGLLANVTAL RILDLSNNQL KTLPLDLLRG PLQLERLHLE GNRLQVLEEG LLAPQPGLRY LFLSDNKLAA VAAGAFRGLQ QLDMLDLSNN SLTSVPKGLW TSLGQPTRDM EDGFDISGNP WICDHNLDDL YRWLVANKDK MFSRNDTRCA RPEALKGQTL LEAAESH.

    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

    Lrg1 Canine

About LRG1 / Leucine-rich alpha-2-glycoprotein 1:

LRG1 (leucine-rich alpha-2-glycoprotein 1) is a protein that is encoded by the gene LRG1. Proteins in the leucine-rich repeat (LRR) family, including LRG1, involve protein-protein interaction, signal transduction, cell adhesion, and development. During granulocyte differentiation, LRG1 is expressed. 

LRG1 Function
LRG1 has been shown to promote neovascularization (new blood vessel growth) in endothelial cells by causing a switch in transforming growth factor-beta (TGFbeta) signalling. LRG1 binds to the endoglin accessory receptor and promotes signalling through the ALK1-Smad1/5/8 pathway.

Leucine-rich alpha-2-glycoprotein 1 Mechanism
Proteins in the leucine-rich repeat (LRR) family, including LRG1, involve protein-protein interaction, signal transduction, cell adhesion, and development. During granulocyte differentiation, LRG1 is expressed.
O'Donnell et al. (2002) observed expression of mouse Lrg1 during G CSF-induced granulocytic differentiation of 32DCL3G cells as early as 16 hours after GCSF induction, with expression increasing 80-fold in 5 days. When the time course of Lrg1 induction was compared to that of other genes upregulated during neutrophilic granulocyte differentiation, it was discovered that Lrg1 expression occurs early in the process.
The upregulation of LRG1 expression was associated with DMSO-induced granulocytic differentiation of human promyelocytic leukemia HL-60 cells. Increased LRG1 expression was also found in G CSF-treated human cells derived from a myeloproliferative disorder patient. In contrast, LRG1 expression was found to be lower after PMA treatment and the induction of monocytic differentiation in HL-60 cells.

LRG1 Diagnostic aid
Because LRG protein levels are markedly elevated in acute appendicitis, it could be used as a diagnostic aid. LRG1 could be a promising therapeutic target for the treatment of diseases characterized by abnormal neovascularization.