Klotho is a type-I membrane protein which is related to beta-glucosidases. Reduced production of the Klotho protein is detected in patients with chronic renal failure (CRF), which may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. In addition, mutations within the Klotho protein are linked with ageing and bone loss.
The Klotho Human Recombinant is produced in CHO cells and fused with a C-terminal 6xHis Tag. The Klotho His Tagged Fusion Protein is 59.5kDa protein containing a total of 522 amino acid residues. Klotho protein is isoform 2 (UniProt entry Q9UEF7–2 Isoform 2) known as secreted.
CHO (Chinese Hamster Ovarian Cells).
Klotho Human recombinant was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.08M phosphate buffer and 0.1M NaCl, pH 7.2.
It is recommended to add to Klothodeionized water to prepare a working stock solution of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter Klothoby an appropriate sterile filter before using it in the cell culture.
Store lyophilized Klotho Human recombinant at -20°C. Aliquot Klotho after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Amino acid sequence
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYHHHHH HH.
Greater than 90% as determined by SDS-PAGE.
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