Kallikrein-11 isoform 1, KLK11, PRSS20, TLSP.
Kallikreins are a subgroup of serine proteases having various physiological functions. Numerous kallikreins are involved in carcinogenesis. Kallikrein-11 (KLK11) which is a multifunctional protease is 1 of the 15 kallikrein subfamily members found in a cluster on chromosome 19. KLK11 cleaves synthetic peptides after arginine but not lysine residues.
KLK11 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 241 amino acids (19-250a.a.) and having a molecular mass of 26.7 kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
KLK11 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Sf9, Insect cells.
KLK11 protein solution ( 0.5mg/m ) contains 50mM Tris-HCl (pH 7.5), 0.1M NaCl, 2mM CaCl2 and 10% glycerol.
Avoid multiple freeze-thaw cycles.
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADLETRIIKG FECKPHSQPW QAALFEKTRL LCGATLIAPR WLLTAAHCLK PRYIVHLGQH NLQKEEGCEQ TRTATESFPH PGFNNSLPNK DHRNDIMLVK MASPVSITWA VRPLTLSSRC VTAGTSCLIS GWGSTSSPQL RLPHTLRCAN ITIIEHQKCE NAYPGNITDT MVCASVQEGG KDSCQGDSGG PLVCNQSLQG IISWGQDPCA ITRKPGVYTK VCKYVDWIQE TMKNNHHHHH H.