Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I IFNs.
IFN-gamma Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized IFN gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
1. UV spectroscopy at 280 nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IFN-g as a Reference Standard.
Safety Data Sheet
1.Title:Candida albicans abrogates the expression of IFN-G-inducible protein-10 in human keratinocytes.
Publication:Article first published online: 18 JUL 2008DOI: 10.1111/j.1574-695X.2008.00457.x
Link:IFN g prospec publication
Publications: Antimicrobial agents and chemotherapy 50.1 (2006): 396-398
Link: IFN g Human prospec publication
Publications: PloS one 8.12 (2013): e82867
Link: IFN-Gamma prospec publication
Publications: Scandinavian journal of immunology 68.3 (2008): 287-296
Link: IFN-Gamma Human prospec publication
Publication: Cancer research 68.6 (2008): 2006-2013.
Link: IFN-gamma prospec publication
Publication: Clinical & Experimental Immunology 162.3 (2010): 460-473.
Link: IFNG prospec publication