prospec
IL 2 Rat

IL 2 Rat

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  • Description
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  • IL 2 Rat

  • Interleukin-2 Rat Recombinant
  • CYT-382
  • Shipped with Ice Packs

Catalogue number

CYT-382

Synonyms

T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Introduction

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description

Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.

The IL-2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

The IL-2 solution contains 0.2µm filtered solution in 20mM PB pH3.5 and 30% Glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.

Store, frozen at -20°C for longer periods of time.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

Purity

Greater than 97.0% as determined by SDS-PAGE and HPLC.

Amino acid sequence

APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.

References

Title:Functional Gap Junctions Facilitate Melanoma Antigen Transfer and Cross-Presentation between Human Dendritic Cells.
Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957
Link:http://www.jimmunol.org/content/178/11/6949.full

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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