T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.
Interleukin-2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 16kDa.
The IL-2 is purified by proprietary chromatographic techniques.
Sterile filtered colorless solution.
The IL-2 solution contains 0.2µm filtered solution in 20mM PB pH3.5 and 30% Glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 97.0% as determined by SDS-PAGE and HPLC.
Amino acid sequence
APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ.
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is less than 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.
Publication:The Journal of Immunology June 1, 2007 vol. 178 no. 11 6949-6957