immediate early response 3, DIF-2, DIF2, GLY96, IEX-1,
IEX-1L, IEX1, PRG1, Protein DIF-2, Immediate early protein GLY96, PACAP-responsive gene 1
protein, Differentiation-dependent gene 2 protein, IER3.
Immediate Early Response 3 (IER3) functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. IER3 has a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. In addition, IER3 serves as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, IER3 provides pancreatic ductal adenocarcinoma with significant resistance to cell stress, such as starvation.
IER3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 105 amino acids (1-82 a.a.) and having a molecular mass of 11.4kDa.
IER3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Sterile Filtered clear solution.
IER3 protein solution (0.25mg/ml) contains 20mM Tris-HCl
buffer, (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMCHSRSC HPTMTILQAP TPAPSTIPGP RRGSGPEIFT FDPLPEPAAA
PAGRPSASRG HRKRSRRVLY PRVVRRQLPV EEPNP.
Safety Data Sheet
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.