prospec

HEXA Human, Sf9

  • Name
  • Description
  • Pricings
  • Quantity
  • HEXA Human, Sf9
  • Hexosaminidase A Human Recombinant, SF9
  • Shipped with Ice Packs

Catalogue number

ENZ-881

Synonyms

Hexosaminidase A (Alpha Polypeptide), N-Acetyl-Beta-Glucosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase Subunit Alpha, Hexosaminidase Subunit A, EC 3.2.1.52, TSD, Beta-Hexosaminidase Subunit Alpha, GM2 Gangliosidosis, Tay Sachs Disease, EC 3.2.1, Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, Hexosaminidase subunit A, N-acetyl-beta-glucosaminidase subunit alpha.

Introduction

HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase which, combined with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules having N-acetyl hexosamines terminus. The two subunits composing Beta-hexosaminidase, alpha and beta, belong to the glycosyl hydrolases family and are encoded by distinct genes. Alpha subunit gene mutations can cause Tay-Sachs disease (GM2-gangliosidosis type I).

Description

HEXA produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 513 amino acids (23-529a.a.) and having a molecular mass of 59.2kDa. 
(Molecular size on SDS-PAGE will appear at approximately 50-70kDa). 
HEXA is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

HEXA protein solution (0. 5mg/ml) contains phosphate buffered saline (pH7.4).

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by SDS-PAGE.

Amino acid sequence

LWPWPQNFQT SDQRYVLYPN NFQFQYDVSS AAQPGCSVLD EAFQRYRDLL FGSGSWPRPY LTGKRHTLEK NVLVVSVVTP GCNQLPTLES VENYTLTIND DQCLLLSETV WGALRGLETF SQLVWKSAEG TFFINKTEIE DFPRFPHRGL LLDTSRHYLP LSSILDTLDV MAYNKLNVFH WHLVDDPSFP YESFTFPELM RKGSYNPVTH IYTAQDVKEV IEYARLRGIR VLAEFDTPGH TLSWGPGIPG LLTPCYSGSE PSGTFGPVNP SLNNTYEFMS TFFLEVSSVF PDFYLHLGGD EVDFTCWKSN PEIQDFMRKK GFGEDFKQLE SFYIQTLLDI VSSYGKGYVV WQEVFDNKVK IQPDTIIQVW REDIPVNYMK ELELVTKAGF RALLSAPWYL NRISYGPDWK DFYIVEPLAF EGTPEQKALV IGGEACMWGE YVDNTNLVPR LWPRAGAVAE RLWSNKLTSD LTFAYERLSH FRCELLRRGV QAQPLNVGFC EQEFEQTHHH HHH.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Back to Top