prospec

GZMB Human

  • Name
  • Description
  • Pricings
  • Quantity
  • GZMB Human
  • Granzyme-B Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-855

Synonyms

Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B.

Introduction

Granzyme-B (GZMB) is essential for target cell lysis in cell-mediated immune responses. GZMB is related to an activation cascade of caspases responsible for apoptosis execution. GZMB cuts caspase-3, -7, -9 and 10 to activate enzymes mediating apoptosis.

Description

GZMB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 248 amino acids (21-247 a.a.) and having a molecular mass of 27.8kDa.
GZMB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

E.coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

GZMB protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MIIGGHEAKP HSRPYMAYLM IWDQKSLKRC GGFLIQDDFV LTAAHCWGSS INVTLGAHNI KEQEPTQQFI PVKRPIPHPA YNPKNFSNDI MLLQLERKAK RTRAVQPLRL PSNKAQVKPG QTCSVAGWGQ TAPLGKHSHT LQEVKMTVQE DRKCESDLRH YYDSTIELCV GDPEIKKTSF KGDSGGPLVC NKVAQGIVSY GRNNGMPPRA CTKVSSFVHW IKKTMKRY.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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