Granzyme B, T-Cell Serine Protease 1-3E, Cathepsin G-Like 1, Granzyme B (Granzyme 2, Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Cytotoxic T-Lymphocyte Proteinase 2, Cytotoxic Serine Protease B , Human Lymphocyte Protein, Fragmentin 2, EC 22.214.171.124, CTSGL1, CTLA1 , CSPB , CGL1, SECT , C11, HLP , Cytotoxic T-Lymphocyte-Associated Serine Esterase 1, Lymphocyte Protease, Fragmentin-2 , Granzyme 2 , Granzyme-2 , EC 3.4.21, CTLA-1 , CGL-1, CSP-B, CCPI , GRB.
Granzyme-B (GZMB) is essential for target cell lysis in cell-mediated immune responses. GZMB is related to an activation cascade of caspases responsible for apoptosis execution. GZMB cuts caspase-3, -7, -9 and 10 to activate enzymes mediating apoptosis.
GZMB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247a.a.) and having a molecular mass of 26.5kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). GZMB is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
GZMB protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) ,20% glycerol and 1mM DTT.
Greater than 95.0% as determined by SDS-PAGE.
Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
Amino acid sequence
GEIIGGHEAK PHSRPYMAYL MIWDQKSLKR CGGFLIRDDF VLTAAHCWGS SINVTLGAHN IKEQEPTQQF IPVKRPIPHP AYNPKNFSND IMLLQLERKA KRTRAVQPLR LPSNKAQVKP GQTCSVAGWG QTAPLGKHSH TLQEVKMTVQ EDRKCESDLR HYYDSTIELC VGDPEIKKTS FKGDSGGPLV CNKVAQGIVS YGRNNGMPPR ACTKVSSFVH WIKKTMKRYH HHHHH