Glycinin Ara h 3 is a seed storage protein, 11 S globulin and trypsin inhibitor from peanut. Each subunit of the hexamer is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond. Ara h 3 and Ara h 4 are isoforms. Glycinin is the source of sulfur-containing amino acids in seed meals and it exists in the seeds of many leguminous and non-leguminous plants.
Recombinant Glycinin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 60,069 Dalton.
Glycinin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Glycinin is supplied in 20mM HEPES buffer pH-7.9 and 6M Urea.
Avoid multiple freeze-thaw cycles.
2. Immunodot test with positive/negative sera panels.