Gliadin is a common substrate of transglutaminase, which generates neo-epitopes by deamidation of glutamine side chains. In the past, serologic tests for gliadin antibodies usually were not very precise and were not enough for accurate diagnosis due to missing deamidated epitopes within the authentic gliadin fraction traditionally used in diagnostic test kits. ProSpec's deamidated Gliadin isoform matches to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation.
The native Gliadin Triticum Aestivum Grain is purified from wheat by protein chemical methods.
Sterile Filtered clear solution.
Gliadin is supplied in 20mM HEPES buffer pH-7.4 and 6M Urea.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 95.0% as determined by SDS-PAGE.
1. Binds IgG and IgA-type human auto antibodies in sera of patients diagnosed with celiac disease.
2. Immunodot analysis with positive/negative samples.
Western blot with patient sample.