- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2551
Synonyms
Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, FRZB, FIZ, FRE, FRP, FRZB1, SFRP3, OS1, FZRB, hFIZ, FRP-3, SFRP3, SRFP3, FRZB-PEN.
Introduction
Frizzled-Related Protein (FRZB) is a secreted protein which is involved in the regulation of bone development. FRZB gene defects cause the female-specific osteoarthritis (OA) susceptibility. sFRPS (Soluble frizzled-related proteins) serve as modulators of Wnt signaling by direct interaction with Wnts. sFRPS have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB is involved in limb skeletogenesis. FRZB regulates chondrocyte maturation and long bone development.
Description
FRZB produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 302 amino acids (33-325 a.a.) and having a molecular mass of 34.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
FRZB is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The FRZB solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
ADPAACEPVR IPLCKSLPWN MTKMPNHLHH STQANAILAI EQFEGLLGTH CSPDLLFFLC AMYAPICTID FQHEPIKPCK SVCERARQGC EPILIKYRHS WPENLACEEL PVYDRGVCIS PEAIVTADGA DFPMDSSNGN CRGASSERCK CKPIRATQKT YFRNNYNYVI RAKVKEIKTK CHDVTAVVEV KEILKSSLVN IPRDTVNLYT SSGCLCPPLN VNEEYIIMGY EDEERSRLLL VEGSIAEKWK DRLGKKVKRW DMKLRHLGLS KSDSSNSDST QSQKSGRNSN PRQARNHHHH HH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.