Listeria monocytogenes is a pathogenic bacteria that manufactures flagella.At 37 °C inside humans, it causes MogR to repress the expression of flagellar proteins, thus stopping the manufacturing of flagella. Though, in the low-temperatures externally of the host, the GmaR inactivates MogR, enabling flagellar formation.
Flagellin Listeria Monocytogenes Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of approximately 31.2kDa. The Flagellin FLA is fused to a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Sterile Filtered colorless solution.
The Flagellin Listeria Monocytogenes 1mg/ml solution contains 10mM PBS (pH-7.2).
Lyophilized Flagellin FLA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flagellin should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Greater than 90% as determined by SDS-PAGE.