HBGF-2, Prostatropin, FGF-2, FGB-b.
FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
The binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Amino acid sequence
1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard.
1. Title: Fibroblast Growth Factor-2 Autofeedback Regulation in Pituitary
Folliculostellate TtT/GF Cells
Publication: Endocrinology 150.7 (2009): 3252-3258.
2. Title: The Mouse Cornea as a Transplantation
Site for Live Imaging of Engineered Tissue Constructs
Publication: Cold Spring Harbor Protocols 2010.4 (2010): pdb-prot5416.