prospec

DNAJB8 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • DNAJB8 Human
  • DnaJ (Hsp40) Homolog, Subfamily B, Member 8 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

HSP-050

Synonyms

DnaJ homolog subfamily B member 8, DNAJB8, DJ6.

Introduction

DnaJ homolog subfamily B member 8 (DNAJB8) is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins.DNAJB8 is a member of the DNAJ/HSP40 family of proteins that regulate chaperone activity. DNAJB8 is (de)acetylated at 2 conserved C-terminal lysines which aren’t involved in substrate binding, but do have a role in suppressing protein aggregation. DNAJB8 has been implicated as a cancer-testis antigen and as a cancer stem-like cell antigen involved in renal cell carcinoma.

Description

DNAJB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232 a.a.) and having a molecular mass of 27.8kDa.
DNAJB8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

The DNAJB8 solution (0.25mg/ml) 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Avoid multiple freeze-thaw cycles.

Purity

Greater than 90% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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