prospec

DsbC

  • Name
  • Description
  • Pricings
  • Quantity
  • DsbC
  • Disulfide-Bond Isomerase Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-291

Synonyms

DsbC, Thiol:disulfide interchange protein dsbC.

Introduction

Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones.

Description

Disulfide-Bond Isomerase Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 216 amino acids (21-236) and having a molecular mass of 23.5 kDa.
DsbC is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

1 mg/ml solution containing 20mM phosphate buffer pH 7.5 and 20mM EDTA.

Amino acid sequence

DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPVNVTNKMLLKQ LNALEKEMIV YKAPQEKHVI VFTDITCGY CHKLHEQMAD YNALGITVRYLAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YVLGVQLGVSGTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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