- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
ENZ-291
Synonyms
Disulfide-bond isomerase C, dsbC, xprA, Disulfide-bond isomerase (DsbC) E.Coli, Thiol:disulfide interchange protein dsbC, Disulfide-bond isomerase C Thiol:disulfide interchange protein dsbC .
Introduction
Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones.
Description
Disulfide-Bond Isomerase Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids (21-236) and having a molecular mass of 23.6 kDa.
DsbC is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
1 mg/ml solution containing 20mM Tris-HCl buffer pH 7.5 and 2mM EDTA.
Amino acid sequence
MDDAAIQQTL AKMGIKSSDI QPAPVAGMKT VLTNSGVLYI TDDGKHIIQG PMYDVSGTAP VNVTNKMLLK QLNALEKEMI VYKAPQEKHV ITVFTDITCG YCHKLHEQMA DYNALGITVR YLAFPRQGLD SDAEKEMKAI WCAKDKNKAF DDVMAGKSVA PASCDVDIAD HYALGVQLGV SGTPAVVLSN GTLVPGYQPP KEMKEFLDEH QKMTSGK.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.