nsLTP Cor a 8, LTP Cor a 8.
Nonspecific lipid transfer proteins (nsLTPs) are expressed throughout the plant tissues. They have a typical cysteine pattern forming disulfide bonds and are important allergens in fruits and tree nuts. Sensitization to nsLTP Cor a 8.0101 is more frequent among patients with hazelnut allergy in the Mediterranean area than the northern parts of Europe.
Recombinant Non-specific Lipid-Transfer Protein Cor a 8 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 11kDa.
Cor a 8.0101 is expressed with a 10xHis tag and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Cor a 8.0101 is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgE-type human antibodies.
2. Immunodot test with positive/negative samples
Tested by LAL (Limulus Amoebocyte Lysate) chromogenic endotoxin assay.