This occurs during a myocardial infarct (MI or heart attack) when CK-MB Type II is released from damaged heart muscle, and the C-terminal lysine is cleaved in the blood stream, thus creating CK-MB Type I. This difference can be exploited in diagnosis of an MI.
Each mg of protein contains 10mM Tris-HCl, pH-6.8, 0.5mM EDTA and 0.5mM DTT, 50% (v/v) glycerol.
CKMBITII although stable at 15°C for 7 days, should be stored below -18°C.
Please prevent freeze-thaw cycles.
The enzyme activity measured by kinetic assay at 340nm was 650 IU/mg at 37 degrees.