- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
PRO-2618
Synonyms
B3GAT1, CD57, GLCATP, GLCUATP, HNK1, LEU7, NK-1, NK1, Beta-1,3-glucuronyltransferase 1, Glucuronosyltransferase P, GlcAT-P, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase, GlcUAT-P.
Introduction
CD57 also called B3GAT1is part of the glucuronyltransferase gene family. CD57 exhibits strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. CD57 takes part in the glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1.
Description
CD57 Human Recombinant produced in Baculovirus is a single, glycosylated, polypeptide chain containing 549 amino acids (28-334aa) and having a molecular mass of 62.3kDa.
CD57 is fused to a 239 amino acid hIgG-His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The CD57 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 10% glycerol and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
ADPTLAPLLA VHKDEGSDPR RETPPGADPR EYCTSDRDIV EVVRTEYVYT RPPPWSDTLP
TIHVVTPTYS RPVQKAELTR MANTLLHVPN LHWLVVEDAP RRTPLTARLL RDTGLNYTHL
HVETPRNYKL RGDARDPRIP RGTMQRNLAL RWLRETFPRN SSQPGVVYFA DDDNTYSLEL
FEEMRSTRRV SVWPVAFVGG LRYEAPRVNG AGKVVGWKTV FDPHRPFAID MAGFAVNLRL
ILQRSQAYFK LRGVKGGYQE SSLLRELVTL NDLEPKAANC TKILVWHTRT EKPVLVNEGK
KGFTDPSVEI LEPKSCDKTH TCPPCPAPEL LGGPSVFLFP PKPKDTLMIS RTPEVTCVVV
DVSHEDPEVK FNWYVDGVEV HNAKTKPREE QYNSTYRVVS VLTVLHQDWL NGKEYKCKVS
NKALPAPIEK TISKAKGQPR EPQVYTLPPS RDELTKNQVS LTCLVKGFYP SDIAVEWESN
GQPENNYKTT PPVLDSDGSF FLYSKLTVDK SRWQQGNVFS CSVMHEALHN HYTQKSLSLS PGKHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.