prospec

CD247 Human, Sf9

  • Name
  • Description
  • Pricings
  • Quantity
  • CD247 Human, Sf9
  • CD247 Human Recombinant, Sf9
  • Shipped with Ice Packs

Catalogue number

PRO-2529

Synonyms

CD247 Molecule, T-Cell Surface Glycoprotein CD3 Zeta Chain, T-Cell Receptor T3 Zeta Chain, CD247 Antigen, CD3Z, TCRZ, T3Z, T-Cell Antigen Receptor Complex, Zeta Subunit Of CD3, CD3Z Antigen, Zeta Polypeptide (TiT3 Complex), CD3z Antigen, Zeta Polypeptide (TiT3 Complex) , CD247 Antigen, Zeta Subunit , TCR Zeta Chain, CD3zeta Chain, CD3-ZETA, IMD25, CD3H, CD3Q.    

Introduction

T-cell surface glycoprotein CD3 zeta chain (CD247) is a member of the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which along with T-cell receptor alpha/beta and gamma/delta heterodimers, and also with CD3-gamma, -delta and -epsilon, creates the T-cell receptor-CD3 complex. The zeta chain has a central role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the CD247 antigen causes impaired immune response.

Description

CD247 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 122 amino acids (52-164a.a.) and having a molecular mass of 14.1kDa. (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa). CD247 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Source

Sf9, Baculovirus cells.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

CD247 protein solution (0.2mg/ml) contains 20mM Tris-HCl buffer (pH 6.8), 50% glycerol, 1mM DTT, 1mM EDTA and  0.1M NaCl.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. 
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). 
Avoid multiple freeze-thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Amino acid sequence

ADPRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPRHHHH HH

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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