prospec

CD14 Antibody

  • Name
  • Description
  • Pricings
  • Quantity
  • CD14 Antibody
  • CD14, Mouse Anti-Human
  • Shipped at Room temp.

Catalogue number

ANT-251

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein.

Type

Mouse Anti Human Monoclonal.

Introduction

CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.

Clone

hCD14.

Immunogen

Purified human B Cells Ig SubclassMouse IgG1.

Purification Method

Ion exchange column.

Formulation

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Solubility

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Applications

Blocking and staining antibody. For staining, use 10µl/1,000,000 cells. Titer for blocking LPS binding should be determined by the investigator.

Available Conjugates

This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10µl/106 cells.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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