prospec

Carbonic Anhydrase 2 Human

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  • Description
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  • Quantity
  • Carbonic Anhydrase 2 Human
  • Carbonic Anhydrase 2 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-420

Synonyms

Carbonic anhydrase 2, Carbonate dehydratase 2, Carbonic Anhydrase II, CA-II, Carbonic anhydrase C, CAC, CA2, CAII, Car2.

Introduction

The enzyme Carbonic anhydrase II having an accession number of NP_414668 is also called carbonate dehydratase which is part of the enzyme family that catalyses rapid inter-conversion of carbon dioxide & water to bicarbonate, carbonic acid and protons (CO2 + H2O ? HCO3? + H+), a reaction that occurs rather slowly in the absence of a catalyst. The majority of carbonic anhydrases enclose a zinc ion in their active site and therefore is classified as metalloenzymes.
The most important function of Carbonic anhydrase is known to preserve acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Carbonic anhydrase has 3 different classes: alpha, beta and gamma which share very little sequence or structural similarity, thus far they all perform the same function and require a zinc ion at the active site. Mammalian carbonic anhydrase is monomeric and belongs to the alpha class. Plant carbonic anhydrase is dimeric and belongs to the beta class.
Methane-producing bacteria carbonic anhydrase is trimeric and grows in hot springs which forms the gamma class.

Description

Carbonic anhydrase 2 Human Recombinant protein produced in E.Coli containing 260 amino acids (1-260) and having a molecular mass of 29.2 kDa.
The Carbonic anhydrase 2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless solution.

Formulation

Carbonic Anhydrase 2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 50mM NaCl and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Amino acid sequence

MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Biological Activity

Specific activity is 50-70 nmoles/min/µg and was obtained by measuring the increase in the amount of p-nitrophenol by its esterase activity. Specific activity is defined as the amount of 
p-nitrophenol that 1ug of enzyme can reduce at 25C for 1 minute.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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