Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP.
Carbonic Anhydrase VIII or CA8 was previously called CA-related protein due to its sequence resemblance to additional recognized carbonic anhydrase genes. Nonetheless CA8 doesn’t have carbonic anhydrase function. This protein keeps bearing a carbonic anhydrase classification because of coherent sequence similarity to additional proteins in carbonic anhydrase family. Mutations in this protein may lead to cerebellar dysequilibrium syndrome type 3 or ataxia mental retardation.
CA8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-290) and having a molecular mass of 35.5kDa.
CA8 Humanis fused to a 24 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
CA8 Human protein (1mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
Greater than 90.0% as determined by SDS-PAGE.
Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE
WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK
SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA
VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW
VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ