prospec

BUB3 Human

  • Name
  • Description
  • Pricings
  • Quantity
  • BUB3 Human
  • BUB3 Human Recombinant
  • Shipped with Ice Packs

Catalogue number

PRO-023

Synonyms

BUB3L, hBUB3, Mitotic checkpoint protein BUB3.

Introduction

Mitotic checkpoint protein BUB3,( BUB3), is a saved component of the mitotic spindle assembly complex (MCC). The encoded protein has 4 WD repeat domains and has a similar sequence as the yeast BUB3 protein. BUB3 is vital for the kinetochore localization of BUB1 and BUBR1. BUB3 participates in the central spindle checkpoint pathway that operates during early embryogenesis. Furthermore BUB3 has a part in regulating the establishment of correct kinetochore-microtubule attachments.

Description

BUB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (1-328 a.a.) and having a molecular mass of 39.5kDa.BUB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

BUB3 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSMTGSNEF KLNQPPEDGI SSVKFSPNTS QFLLVSSWDT SVRLYDVPAN SMRLKYQHTG AVLDCAFYDP THAWSGGLDH QLKMHDLNTD QENLVGTHDA PIRCVEYCPE VNVMVTGSWD QTVKLWDPRT PCNAGTFSQP EKVYTLSVSG DRLIVGTAGR RVLVWDLRNM GYVQQRRESS LKYQTRCIRA FPNKQGYVLS SIEGRVAVEY LDPSPEVQKK KYAFKCHRLK ENNIEQIYPV NAISFHNIHN TFATGGSDGF VNIWDPFNKK RLCQFHRYPT SIASLAFSND GTTLAIASSY MYEMDDTEHP EDGIFIRQVT DAETKPKSPC T.

Safety Data Sheet

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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