Polcalcin Bet v 4, Calcium-binding pollen allergen Bet v 4, Bet v 4, BETV4.
BETV4 is primarily expressed in mature birch (Betula verrucosa) pollen and Causes an allergic reaction in human. BETV4 is a calcium-binding protein of 2-EF-hand type, which is exists in pollen of various plant species. This cross-reactivity can serve as a marker allergen for plant polysensitization.
Recombinant BETV4 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 10,473 Dalton.
BETV4 is expressed with a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
BETV4 is supplied in 20mM HEPES buffer pH-7.6, 200mM NaCl and 20% glycerol.
Avoid multiple freeze-thaw cycles.
2. Immunodot test with positive/negative sera panels.