Bcl 2 Human (minus BH1 domain)

Bcl 2 Human (minus BH1 domain)

B Cell Lymphoma

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  • Name :

    Bcl 2 Human (minus BH1 domain)

    Description:

    B-Cell Leukemia/Lymphoma 2 Human Recombinant (–BH1)

    Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.

    Product # :

    PRO-631

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    Shipped at Room temp

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Description

Bcl-2 Des BH1 domain (136-155 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids 1-135 and 156-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Formulation

The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

More Info

  • Introduction

    BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

  • Synonyms

    Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.

  • Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

  • Stability

    Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

  • Solubility

    Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C.
    Dilute 10 fold into selected buffer system.
    BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.

  • Applications

    Input marker or positive control (Western Blotting).
    Function study.

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Bcl2 Human Des Bh1

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