Ara h 8 allergen.
Ara h 8.0101 which is a part of the PR-10 protein family is a Bet v 1-homologous panallergen. Ara h 8.0101 is cross-reactive with Gly m 4 from soy bean and Bet v 1 from silver birch. Ara h 8.0101 is responsible for oral allergy syndrome and also has low stability to roasting and no stability to gastric digestion.
Recombinant Ara h 8.0101 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 18,192 Dalton.
Ara h 8.0101 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Ara h 8.0101 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Avoid multiple freeze-thaw cycles.
2. Immunodot test with positive/negative sera panels.