prospec
ACE2 (18-740) Human

ACE2 (18-740) Human

  • Name
  • Description
  • Cat#
  • Pricings
  • Quantity
  • ACE2 (18-740) Human

  • Angiotensin Converting Enzyme 2 (18-740 a.a.), Human Recombinant
  • ENZ-1125
  • Shipped with Ice Packs

Catalogue number

ENZ-1125

Introduction

ACE-2 (Angiotensin converting enzyme 2) an enzyme bound to cell membranes in various organs such as intestines arteries , lungs, heart & kidney. ACE2 an entry receptor of SARS coronaviruses as well as SARS-CoV-2,.The coronavirus spike (S) glycoprotein is a class I viral fusion antigen located on the external envelope of the virion that takes part in a critical part in viral infection by identifying host cell receptors and facilitating fusion of the viral and cellular membranes. 2 main domains in coronavirus S1 have been recognized, the N-terminal domain and C-terminal domain. One or the other and/or both S1 domains function as a receptor-binding domain.  SARS-CoV + MERS-CoV equally use C-domain to attach their receptors.ACE2 is a type I transmembrane antigen with an extracellular N-terminal domain having the catalytic site and an intracellular C-terminal tail. ACE2 obtains a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing an HEXXH zinc-binding domain. ACE-2 plays a role as a mono-carboxypeptidase which degrades Ang I to produce the nonapeptide Ang 1–9 and Ang II to create the heptapeptide Ang 1–7.

Description

The HEK293 derived ACE2 Human recombinant protein contains the amino acids 18-740 fused to His tag at N-terminal. ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.

Source

HEK293 Cells

Purification Method

Purified by Metal-Afinity chromatographic technique.

Physical Appearance

Sterile Filtered clear solution.

Formulation

ACE2 Human protein solution is supplied in 50mM Tris-HCl, pH7.5, and 150mM NaCl and glycerol.

Stability

ACE-2 Human Recombinant Protein is shipped on ice packs. Upon arrival, Store at -20°C.

Purity

Protein is >95% pure as determined SDS-PAGE.

Biological Activity

ACE2 activity was measured by its binding ability in a functional ELISA.

The immobilized Recombinant Human ACE2 protein binds to SARS CoV2 Spike protein Receptor Binding Domain at 2ug per ml.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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