ACE-2 (Angiotensin converting enzyme 2) an enzyme bound to cell membranes in various organs such as intestines arteries , lungs, heart & kidney. ACE2 an entry receptor of SARS coronaviruses as well as SARS-CoV-2,.The coronavirus spike (S) glycoprotein is a class I viral fusion antigen located on the external envelope of the virion that takes part in a critical part in viral infection by identifying host cell receptors and facilitating fusion of the viral and cellular membranes. 2 main domains in coronavirus S1 have been recognized, the N-terminal domain and C-terminal domain. One or the other and/or both S1 domains function as a receptor-binding domain. SARS-CoV + MERS-CoV equally use C-domain to attach their receptors.ACE2 is a type I transmembrane antigen with an extracellular N-terminal domain having the catalytic site and an intracellular C-terminal tail. ACE2 obtains a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing an HEXXH zinc-binding domain. ACE-2 plays a role as a mono-carboxypeptidase which degrades Ang I to produce the nonapeptide Ang 1–9 and Ang II to create the heptapeptide Ang 1–7.
The HEK293 derived ACE2 Human recombinant protein contains the amino acids 18-740 fused to IgG-His tag ( 242 a.a. ) at N-terminal having a total Mw of 110.8 kDa. ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.
ACE2 Human protein 0.5mg/ml solution is supplied in PBS, pH7.4, and 10% glycerol.
ACE-2 Human Recombinant Protein is shipped on ice packs. Upon arrival, Store at -20°C.
Protein is >90% pure as determined SDS-PAGE.
Determined by its binding ability in a functional ELISA using SARS-CoV-2 Spike RBD. The ED50 range ≤ 100ng/ml. Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH / min. at pH-7.5, at 25C
Amino acid sequence
DGS QSTIEEQ AKTFLDKFNH EAEDLFYQSS LASWNYNTNI TEENVQNMNN AGDKWSAFLK EQSTLAQMYP LQEIQNLTVK LQLQALQQNG SSVLSEDKSK RLNTILNTMS TIYSTGKVCN PDNPQECLLL EPGLNEIMAN SLDYNERLWA WESWRSEVGK QLRPLYEEYV VLKNEMARAN HYEDYGDYWR GDYEVNGVDG YDYSRGQLIE DVEHTFEEIK PLYEHLHAYV RAKLMNAYPS YISPIGCLPA HLLGDMWGRF WTNLYSLTVP FGQKPNIDVT DAMVDQAWDA QRIFKEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQPFLLRN GANEGFHEAV GEIMSLSAAT PKHLKSIGLL SPDFQEDNET EINFLLKQAL TIVGTLPFTY MLEKWRWMVF KGEIPKDQWM KKWWEMKREI VGVVEPVPHD ETYCDPASLF HVSNDYSFIR YYTRTLYQFQ FQEALCQAAK HEGPLHKCDI SNSTEAGQKL FNMLRLGKSE PWTLALENVV GAKNMNVRPL LNYFEPLFTW LKDQNKNSFV GWSTDWSPYA DQSIKVRISL KSALGDKAYE WNDNEMYLFR SSVAYAMRQY FLKVKNQMIL FGEEDVRVAN LKPRISFNFF VTAPKNVSDI IPRTEVEKAI RMSRSRINDA FRLNDNSLEF LGIQPTLGPP NQPPVSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSHEDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQVYTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVMHEALHNHYTQ KSLSLSPGKH HHHHH