prospec

ACTA1 Antibody

  • Name
  • Description
  • Pricings
  • Quantity
  • ACTA1 Antibody
  • Actin, Mouse Anti Human
  • Shipped with Ice Packs

Catalogue number

ANT-749

Synonyms

ACTA, Actin, actin, alpha 1, skeletal muscle, alpha skeletal muscle actin, alpha skeletal muscle, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3.

Type

Mouse Anti Human Monoclonal.

Introduction

Actin is one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans .Actin or ACTA is aprotein localized in the I band of the myofibrils in the muscle, Acting together with myosin.Actin takes part in the contraction and relaxation of muscle. Actin exists as a monomer in low salt concentrations, but filaments form rapidly as salt concentration rises, with the consequent hydrolysis of ATP.Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or magnesium ions, as well as several low affinity sites. It occurs in globular (G-actin) and fibrous (F-actin) forms. Actin is found in all eukaryotic cells (except for nematode sperm). Its other activitiessuch as cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Clone

PAT2F5AT.

Physical Appearance

Sterile filtered colorless solution.

Immunogen

Anti-human ACTA1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACTA1 amino acids 13-377 purified from E. coli.

Ig Subclass

Mouse IgG2a heavy chain and κ light chain.

Purification Method

ACTA1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Formulation

1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.

Storage Procedures

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. 
Prevent freeze thaw cycles.

Stability / Shelf Life

12 months at -20°C. 1 month at 4°C.

Applications

ACTA1 antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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