prospec
ACP2 Human

ACP2 Human

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  • ACP2 Human

  • Acid Phosphatase-2 Human Recombinant
  • ENZ-849
  • Shipped with Ice Packs

Catalogue number

ENZ-849

Synonyms

Acid Phosphatase 2, Lysosoma, EC 3.1.3.2 LAP, Lysosomal Acid Phosphatase, ACP2.

Introduction

Acid Phosphatase-2, also known as ACP2 is composed of two subunits, Alpha & beta, and is chemically as well as genetically distinct from red cell acid phosphatase. ACP2 belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. In addition, Acid phosphatase deficiency is caused by mutations in the ACP2-beta subunit as well as ACP3-alpha subunit genes.

Description

ACP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 373 amino acids (31-380 a.a.) and having a molecular mass of 42.9kDa.

ACP2 is fused to a 23 amino acid His-Tag at N-Terminus and purified by conventional chromatography techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile filtered colorless solution.

Formulation

ACP2 protein solution (1mg/ml) contains 20mM Tris-HCl (pH8.0) and 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MGSRSLRFVT LLYRHGDRSP VKTYPKDPYQ EEEWPQGFGQ LTKEGMLQHW ELGQALRQRY HGFLNTSYHR QEVYVRSTDF DRTLMSAEAN LAGLFPPNGM QRFNPNISWQ PIPVHTVPIT EDRLLKFPLG PCPRYEQLQN ETRQTPEYQN ESSRNAQFLD MVANETGLTD LTLETVWNVY DTLFCEQTHG LRLPPWASPQ TMQRLSRLKD FSFRFLFGIY QQAEKARLQG GVLLAQIRKN LTLMATTSQL PKLLVYSAHD TTLVALQMAL DVYNGEQAPY ASCHIFELYQ EDSGNFSVEM YFRNESDKAP WPLSLPGCPH RCPLQDFLRL TEPVVPKDWQ QECQLASGPA DTE

Safety Data Sheet

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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