Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.
PCNA full length Human Recombinant protein produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 62kDa which includes GST-tag fusion protein.
Sterile Filtered colorless solution.
The PCNA solution contains 50mM Tris-Acetate pH-7.5 & 1mM EDTA & 20% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
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