Monoclonal Antibody
ProSpec’s monoclonal antibodies are produced in hybridoma cell lines derived from the fusion of myeloma cells and antibody-producing B-lymphocytes. We guarantee two positive clones for monoclonal antibodies developed in mouse. For any protein antigen (at least 2.5mg provided by the requestor, > 80% purity in SDS-PAGE) we guarantee two positive clones or more with positive ELISA results. Our prices are on a setup fee basis.
- Service
- Screening Method
- Deliverables
- Price(USD)
- Timeline
- Mouse monoclonal antibody production service
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- ELISA
- ELISA & Western Blot
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- 2 hybridoma clones + purified antibody
- 2 hybridoma clones + purified antibody
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- $9,000
- $13,000
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- 3-6 Month
- 3-6 Month
- Mouse monoclonal antibody production without purification
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- ELISA
- ELISA & Western Blot
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- 2 hybridoma clones + purified antibody
- 2 hybridoma clones + purified antibody
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- $8,000, additional hybridoma $400
- $12,000, additional hybridoma $2,000
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- 3-6 Month
- 3-6 Month
- Protein A/G purification
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- N/A
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- >0.8mg
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- $500/mouse
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- N/A
- Ascites (not purified)
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- N/A
- N/A
- N/A
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- Ascites of 1xmouse (2-5 ml)
- Ascites of 5 mice
- Ascites of 10 mice
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- $400
- $1,800
- $3,600
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- 3 Weeks
- 3 Weeks
- 3 Weeks
Follow Ups
- The above prices are for the production of antibodies using antigens provided by the requestor. However, we also provide a custom protein service at an extra charge, if requested.
- There is a non-refundablesetup charge of $400 prior to the custom monoclonal antibody production. The setup charge is included in the above prices.
- ELISA screening is also performed in (250ng/well) ELISA (general selection standard: OD value > 1.0).
- Western Blot for screening is performed using standard cell or tissue lysates. However, if a specific cell lysates is requested, and we do not have it, the client should provide it.
- Shipping charges are not included. Hybridoma is shipped on dry ice. We charge $250 for dry ice shipments to U.S.A and W.Europe