prospec

HARS Human

  • Name
  • Description
  • Pricings
  • Quantity
  • HARS Human
  • Histidyl-tRNA Synthetase Human Recombinant
  • Shipped with Ice Packs

Catalogue number

ENZ-268

Synonyms

Histidyl-tRNA synthetase, EC 6.1.1.21, Histidine-tRNA ligase, HisRS, HRS, FLJ20491, JO-1, HARS.

Introduction

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Description

Histidyl-tRNA Synthetase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 55 kDa.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered clear solution.

Formulation

The protein solution contains 500mM NaCl and 10mM Tris (pH 8.0) and 6M Urea.

Stability

Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored below -18°C.
Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by Analysis by SDS-PAGE.

Applications

Western Blot: Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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