- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-962
Synonyms
Interleukin 34, C16orf77, MGC34647, IL34.
Introduction
Interleukin 34 (IL34) is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor and is a part of a group of cytokines called interleukins. IL34 increases growth or survival of immune cells known as monocytes. IL34 protein obtains its activity by binding the Colony stimulating factor 1 receptor. Interleukin 34 has also a potential part in viral infection, the adaptive immune response and bone marrow cell proliferation. Furthermore, IL34 plays an important role in innate immunity and in inflammatory processes.
Description
Interleukin 34 Human Recombinant produced in HEK cells is a glycosylated homodimer, containing 460 amino acids and having a total molecular weight of 52.5kDa.
The IL34 protein is fused to a His-tag at C-terminus & purified by proprietary chromatographic techniques.
The IL34 protein is fused to a His-tag at C-terminus & purified by proprietary chromatographic techniques.
Source
HEK.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The IL34 was lyophilized from a solution containing 20mM Sodium Citrate, pH 3.0 and 75mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-34 in sterile 18M-cm H2O containing 0.1% endotoxin-free recombinant HSA.
Stability
Lyophilized IL34 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Interleukin 34 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
NEPLEMWPLT QNEECTVTGF LRDKLQYRSR LQYMKHYFPI NYKISVPYEG VFRIANVTRL QRAQVSEREL RYLWVLVSLS ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSP QGLTDVEVSP APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCS PEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LPHHHHHHHH.
Biological Activity
The specific activity was measured by its ability to induce secretion of MCP-1 from PBMCs.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.