- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
ENZ-1019
Synonyms
Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIase
F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Rotamase F.
Introduction
PPIF is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIF accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Description
Cyclophilin F Rat Recombinant produced in E.Coli is a
single, non-glycosylated polypeptide chain containing 200 amino acids (30-206 a.a)
and having a molecular mass of 21.2Da.
Cyclophilin F is fused to a
23 amino acid His-tag at N-terminus & purified by proprietary
chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
Cyclophilin
F protein solution (1mg/ml) containing Phosphate Buffered Saline (pH 7.4), 10%
glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Biological Activity
Specific
activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that
cleaves 1nmol of suc-AAFP-PNA per minute at 37C in Tris–HCl pH 8.0 using chymotrypsin.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN
FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP
NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF
GSKSGKTSKK IVITDCGQLS.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.