BMP4, ZYME, BMP2B, BMP2B1.
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
BMP-4 Human Recombinant produced in HEK cells is a glycosylated disulfide linked homodimer, having a total molecular weight of 34kDa.
The BMP-4 is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The BMP-4 was lyophilized from 1.07mg/ml in 2xPBS+6% ethanol.
It is recommended to reconstitute the lyophilized BMP-4 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.
Lyophilized BMP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 95% as obsereved by SDS-PAGE.
Amino acid sequence
GASHASLIPE TGKKKVAEIQ GHAGGRRSGQ SHELLRDFEATLLQMFGLRR RPQPSKSAVI PDYMRDLYRL QSGEEEEEQI HSTGLEYPER PASRANTVRS FHHEEHLENI PGTSENSAFR FLFNLSSIPE NEAISSAELR LFREQVDQGP DWERGFHRIN IYEVMKPPAE VVPGHLITRL LDTRLVHHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG QHVRISRSLP QGSGNWAQLR PLLVTFGHDG RGHALTRRRR AKRSPKHHSQ RARKKNKNCR RHSLYVDFSD VGWNDWIVAP PGYQAFYCHG DCPFPLADHL NSTNHAIVQT LVNSVNSSIP KACCVPTELS AISMLYLDEY DKVVLKNYQE MVVEGCGCR.
The specific activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 2.9ng/ml corresponding to a Specific Activity of 220,750.55IU/mg.
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