tPA Human
Tissue Plasminogen Activator Human Recombinant

Synonyms:
Tissue-type plasminogen activator, EC 3.4.21.68, tPA, t-PA, t-plasminogen activator, Alteplase, Reteplase, TPA, T-PA, DKFZp686I03148.

Introduction:
Tissue plasminogen activator (abbreviated PLAT or tPA) is a secreted serine proteasewhich converts the proenzymeplasminogento plasmin, a fibrinolyticenzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin.
This enzyme plays a role in cell migrationand tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysiswhich can result in thrombosisor embolism.

Description:
Tissue Plasminogen Activator Human Recombinant produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin.
The tPA is purified by proprietary chromatographic techniques.

Source:
Chinese Hamster Ovary Cells (CHO)

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Each mg of Alteplase contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80.

Solubility:
It is recommended to reconstitute the lyophilized t-PA in sterile 18MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Reteplase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution tPA should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Enzymatic Activity:
580,000 IU/mg.

References:
Bachmann, F. and Kruithof, E.,Semin. Thromb. Hemost. 10, 6 (1984) Klausner, A.,Biotechnology 4, 706 (1986) Verheijen, J.H., et al., Bergmeyer, J., and Grassi, M., ed., Methods of Enzymatic Analysis Meth. Enzym. Anal. 3rd ed., (1984),5, 425

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.