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Catalogue Number
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CYT-015
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Synonyms
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Precursor Form Neurotrophin-3, proNT-3.
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Introduction
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proNT-3 (the precursor form of neurotrophin-3) functions through p75NTR and sortilin and induces neuronal apoptosis. In contrast, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. Since axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also has an important role in the mechanism of retrograde signaling.
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Description
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proNT3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4kDa.
The proNT3 is purified by proprietary chromatographic techniques.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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proNT3 was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0 and 300mM NaCl.
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Solubility
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It is recommended to reconstitute the lyophilized proNT3 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
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Stability
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Lyophilized proNT3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution proNT3 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
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Purity
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Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
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Amino acid sequence
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The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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