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Catalogue Number
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WNV-002
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Introduction
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West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
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Description
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The E.Coli derived 20kda recombinant protein contains the West-Nile N-Terminal Pre-M Virus immunodominant regions. The protein is fused with 6xHis tag.
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Method
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Purified by proprietary chromatographic technique.
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Purity
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Protein is >95% pure as determined by 10% PAGE (coomassie staining).
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Formulation
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20mM phosphate buffer pH 7.5.
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Stability
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WNV Pre-M although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
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Specificity
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Immunoreactive with sera of West Nile virus infected individuals.
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Applications
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Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
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Amino Acid Sequence
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MVTLSNFQGKVMMTVNATDVTDVITIPTAAGKNLCIVRA MDVGYLCEDTITYECPVLAAGNDPEDIDCWCTKSSVYVR
YGRCTKTRHSRRSRRSLTVQTHGESTLANKKGAWLDSTK
ATRYLVKTESWILRNPGYALE.
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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Email: info@prospecbio.com |
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