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West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
The E.Coli derived 18kda recombinant protein contains the West-Nile N-rerminus Envelope Virus immunodominant regions. The protein is fused with 6xHis tag.
Purified by proprietary chromatographic technique.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
20mM phosphate buffer pH 7.5.
Protein is shipped at ambient temperature. Upon arrival, Store at
-20°C.
Five years frozen. One month in solution at room temperature.
Immunoreactive with sera of West Nile virus infected individuals.
Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
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