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Catalogue Number
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WNV-001
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Introduction
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West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus; both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDaprotein blocks; the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
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Description
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The E.Coli derived recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused with 6xHis tag. Mw~42kDa.
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Method
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Purified by proprietary chromatographic technique.
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Purity
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Protein is >95% pure as determined by 10% PAGE (coomassie staining).
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Formulation
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(1mg/ml) 20mM Phosphate buffer pH 7.5.
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Storage
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WNV Envelope although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles.
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Specificity
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Immunoreactive with sera of West Nile virus infected individuals.
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Applications
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Antigen in ELISA and Western blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
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Amino Acid Sequence
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MQLKGTTYGV CSKAFKFLGT PADTGHGTVV LELQYTGTDG PCKVPISSVA SLNDLTPVGR LVTVNPFVSV ATANAKVLIE LEPPFGDSYI VVGRGEQQIN HHWHKSGSSI GKAFTTTLKG ALEMQLKGTT YGVCSKAFKF LGTPADTGHG TVVLELQYTG TDGPCKVPIS SVASLNDLTP VGRLVTVNPFV SVATANAKVL IELEPPFGDS YIVVGRGEQQI NHHWHKSGSS IGKAFTTTLK GALEMQLKGT TYGVCSKAFK FLGTPADTGH GTVVLELQYT GTDGPCKVPI SSVASLNDLT PVGRLVTVNP FVSVATANAK VLIELEPPFG DSYIVVGRGE QQINHHWHKS GSSIGKAFTT TLKGALEHHH HHH
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Usage
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ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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