- Name
- Description
- Cat#
- Pricings
- Quantity
Catalogue number
CYT-628
Synonyms
Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB.
Introduction
VEGFB is a growth factor for endothelial cells. VEGFB 167 a.a. binds heparin and neuropilin-1 while the binding to neuropilin-1 of VEGFB 186 a.a. is regulated by proteolysis. Overexpression of VEGFB in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. Increased VEGFB expression is associated with hepatocellular carcinoma. VEGF-B has restricted angiogenic activity in the ischemic heart. VEGFB binds to VEGFR1, Flt1, but not to VEGFR2 and VEGFR3. VEGFB is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis.
Description
VEGFB Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids (22-207) and having a molecular mass of 21.6 kDa (molecular weight on SDS-PAGE will appear higher).
The VEGFB is fused to a 20 amino acid His Tag at N-terminus & purified by proprietary chromatographic techniques.
The VEGFB is fused to a 20 amino acid His Tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless liquid formulation.
Formulation
The VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml corresponding to a specific activity of less than 1,000,000units/mg.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.