VEGFB Human | ProSpec

Description

Our Price

Quantity

Shipping

Vascular Endothelial Growth Factor-B Human Recombinant

	2µg($50)
	10µg($130)
	1mg($5200)

Ice Packs

Catalogue Number

CYT-628

Synonyms

Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB.

Introduction

VEGFB is a growth factor for endothelial cells. VEGFB 167 a.a. binds heparin and neuropilin-1 while the binding to neuropilin-1 of VEGFB 186 a.a. is regulated by proteolysis. Overexpression of VEGFB in mouse heart alters cardiac lipid metabolism and induces myocardial hypertrophy. Increased VEGFB expression is associated with hepatocellular carcinoma. VEGF-B has restricted angiogenic activity in the ischemic heart. VEGFB binds to VEGFR1, Flt1, but not to VEGFR2 and VEGFR3. VEGFB is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis.

Description

VEGFB Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids (22-207) and having a molecular mass of 21.6 kDa.
The VEGFB is fused to 20 amino acid His Tag at N-terminus & purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered colorless liquid formulation.

Formulation

The VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Biological activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml corresponding to a specific activity of less than 1,000,000units/mg.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.