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Catalogue Number
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ENZ-348
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Synonyms
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Uridine phosphorylase, EC 2.4.2.3, UrdPase, UPase, StUP.
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Introduction
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Uridine phosphorylase from Salmonella typhimurium (StUP) catalyzes the reversible phosphorolysis of uridine with the formation of ribose-1-phosphate and uracil.
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Description
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Uridine phosphorylase Salmonella typhimurium Recombinantproduced in E.Coli is a non-glycosylated, polypeptide having a total molecular mass of 163068 Dalton.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered white lyophilized powder.
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Formulation
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The UPase was lyophilized from 1mg/ml solution containing 25mM Tris-HCl, pH 8.0, 0.15M NaCl.
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Solubility
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It is recommended to reconstitute the lyophilized UPase in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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Stability
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Lyophilized UPase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UPase should be stored at 4°C between 2-7 days and for future use below -18°C.
For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
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Purity
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Greater than 95.0% as determined by SDS-PAGE.
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Activity Determination
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One unit phosphorylates 1μm of uridine within 1 min at pH 7.3.
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Enzyme Activity
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30 U/mg protein.
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Usage
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Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Related Products
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